Experimental Investigations: Micromeritic Procedures In Assessing Antinuclear Antibody Patterns In Immunofluorescent Assay
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analyzing patterns of classroom interaction in efl classrooms in iran
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Detection of Antinuclear Antibody in Childhood Rheumatic Diseases by Immunofluorescence Assay and Enzyme Immuno Assay
Background: Anti-nuclear antibodies (ANAs) are specific antibodies directed against a variety of nuclear antigens. Indirect immunofluorecence (ANA-IFA) and Enzyme Immunoassay (ANA-EIA) are commonly used methods for ANA detection. The positivity of ANA-IFA using HEp-2 cell substrate is 90-100% in systemic rheumatic diseases. On the other hand positivity of ANA-EIA is found to be much lower in di...
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The ANA test is an excellent screening test for patients with SLE and a few other connective tissue diseases. The LE cell preparation is an assay that is subjective and costly. Because of the presence of a superior screening test (the ANA) and superior specific auto-antibody tests, the author recommends that the use of LE cell preparations be discontinued. ANA screening tests may be performed e...
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OBJECTIVES To assess possible associations between the presence of antinuclear antibodies (ANAs) and pregnancy outcome in order to determine the significance of this test in obstetric practice. METHODS A case-control study was performed on 408 patients admitted to an obstetric high care unit and on whom ANA testing was consecutively performed. The study group consisted of 46 patients who test...
متن کاملAntinuclear antibody determination in a routine laboratory.
Pitfalls in the method for demonstrating antinuclear antibodies (ANA) by the indirect immunofluorescence technique are described and the use of international standard preparations outlined. Determination of the optimal border dilution dividing positive from negative results is discussed. Each laboratory is a unique setting; it must define its own method, which should rarely be changed. One shou...
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ژورنال
عنوان ژورنال: Folia Medica
سال: 2014
ISSN: 1314-2143
DOI: 10.2478/folmed-2014-0026